7.FMOC-O-叔丁基-L-丝氨酸操作处置与储存：

① 安全操作的注意事项
在有粉尘生成的地方,提供合适的排风设备。
② 安全储存的条件,包括任何不兼容性
贮存在阴凉处。 容器保持紧闭，储存在干燥通风处。
建议的贮存温度： 2 - 8 °C

8.FMOC-O-叔丁基-L-丝氨酸废弃处置：

废物处理方法：
产品
将剩余的和未回收的溶液交给处理公司。
受污染的容器和包装
作为未用过的产品弃置。

9.FMOC-O-叔丁基-L-丝氨酸毒性和生态：

FMOC-O-叔丁基-L-丝氨酸生态学数据:

通常对水是不危害的，若无政府许可，勿将材料排入周围环境。

10.FMOC-O-叔丁基-L-丝氨酸安全信息：

11.FMOC-O-叔丁基-L-丝氨酸制备：

将O-叔丁基-L-丝氨酸悬浮于二氧六环溶液中，与芴甲氧羰基叠氮进行?；从Φ么植罚趐H为9-10的条件下用乙酸乙酯抽提，再经重结晶精制而得。

12.FMOC-O-叔丁基-L-丝氨酸海关：

13.FMOC-O-叔丁基-L-丝氨酸文献：

Hapten-directed spontaneous disulfide shuffling: a universal technology for site-directed covalent coupling of payloads to antibodies.

Stefan Dengl, Eike Hoffmann, Michael Grote, Cornelia Wagner, Olaf Mundigl, Guy Georges, Irmgard Thorey, Kay-Gunnar Stubenrauch, Alexander Bujotzek, Hans-Peter Josel, Sebastian Dziadek, Joerg Benz, Ulrich Brinkmann

文献索引：FASEB J. 29 , 1763-79, (2015)

全文：HTML全文

摘要

Humanized hapten-binding IgGs were designed with an accessible cysteine close to their binding pockets, for specific covalent payload attachment. Individual analyses of known structures of digoxigenin (Dig)- and fluorescein (Fluo) binding antibodies and a new structure of a biotin (Biot)-binder, revealed a "universal" coupling position (52(+2)) in proximity to binding pockets but without contributing to hapten interactions. Payloads that carry a free thiol are positioned on the antibody and covalently linked to it via disulfides. Covalent coupling is achieved and driven toward complete (95-100%) payload occupancy by spontaneous redox shuffling between antibody and payload. Attachment at the universal position works with different haptens, antibodies, and payloads. Examples are the haptens Fluo, Dig, and Biot combined with various fluorescent or peptidic payloads. Disulfide-bonded covalent antibody-payload complexes do not dissociate in vitro and in vivo. Coupling requires the designed cysteine and matching payload thiol because payload or antibody without the Cys/thiol are not linked (<5% nonspecific coupling). Hapten-mediated positioning is necessary as hapten-thiol-payload is only coupled to antibodies that bind matching haptens. Covalent complexes are more stable in vivo than noncovalent counterparts because digoxigeninylated or biotinylated fluorescent payloads without disulfide-linkage are cleared more rapidly in mice (approximately 50% reduced 48 hour serum levels) compared with their covalently linked counterparts. The coupling technology is applicable to many haptens and hapten binding antibodies (confirmed by automated analyses of the structures of 140 additional hapten binding antibodies) and can be applied to modulate the pharmacokinetics of small compounds or peptides. It is also suitable to link payloads in a reduction-releasable manner to tumor- or tissue-targeting delivery vehicles.-Dengl, S., Hoffmann, E., Grote, M., Wagner, C., Mundigl, O., Georges, G., Thorey, I., Stubenrauch, K.-G., Bujotzek, A., Josel, H.-P., Dziadek, S., Benz, J., Brinkmann, U. Hapten-directed spontaneous disulfide shuffling: a universal technology for site-directed covalent coupling of payloads to antibodies. ? The Author(s).

14.FMOC-O-叔丁基-L-丝氨酸英文别名：